DPD spectrophotometry ita ce madaidaicin hanya don gano ragowar chlorine kyauta da jimlar chlorine da aka rage a cikin ma'auni na kasa na kasar Sin "Hanyoyin Ingantattun Kalmomin Ruwa da Hanyoyi na Nazari" GB11898-89, kungiyar Kiwon Lafiyar Jama'a ta Amurka, Kungiyar Ayyukan Ruwa ta Amurka da Hukumar Kula da Gurbacewar Ruwa. Tarayyar A cikin editan "Hanyoyin Gwaji na Standard don Ruwa da Ruwa", an haɓaka hanyar DPD tun bugu na 15 kuma ana ba da shawarar a matsayin daidaitaccen hanyar gwajin chlorine dioxide.
Amfanin hanyar DPD
Yana iya raba chlorine dioxide daga wasu nau'ikan chlorine daban-daban (ciki har da sauran chlorine kyauta, jimlar sauran chlorine da chlorite, da sauransu), yana sauƙaƙa yin gwajin launi. Wannan hanyar ba daidai ba ce kamar titration na amperometric, amma sakamakon ya isa ga yawancin dalilai na gaba ɗaya.
ka'ida
A ƙarƙashin yanayin pH 6.2 ~ 6.5, ClO2 ya fara amsawa tare da DPD don samar da fili mai ja, amma adadin ya bayyana kawai ya kai kashi ɗaya cikin biyar na jimlar adadin chlorine (daidai da rage ClO2 zuwa ions chlorite). Idan samfurin ruwa ya kasance acidified a gaban iodide, chlorite da chlorate suma suna amsawa, kuma lokacin da aka lalata su ta hanyar ƙari na bicarbonate, sakamakon launi ya dace da jimlar chlorine na ClO2. Ana iya hana tsangwama na chlorine kyauta ta ƙara glycine. Tushen shine glycine nan da nan na iya canza chlorine kyauta zuwa aminoacetic acid chlorinated, amma ba shi da tasiri akan ClO2.
Potassium iodate misali stock bayani, 1.006g/L: Auna 1.003g potassium iodate (KIO3, dried a 120 ~ 140 ° C for 2 hours), narke a high-tsarki ruwa, da kuma canja wurin zuwa 1000ml girma.
Zuba gilashin aunawa zuwa alamar kuma a gauraya.
Potassium iodate daidaitaccen bayani, 10.06mg/L: Ɗauki 10.0ml na maganin haja (4.1) a cikin 1000ml volumetric flask, ƙara game da 1g na potassium iodide (4.5), ƙara ruwa don tsarma zuwa alamar, da kuma haɗuwa. Yi shiri a ranar amfani a cikin kwalban ruwan kasa. 1.00ml na wannan daidaitaccen bayani ya ƙunshi 10.06μg KIO3, wanda yayi daidai da 1.00mg/L samuwan chlorine.
Fosphate buffer: Narkar da 24g anhydrous disodium hydrogen phosphate da 46g anhydrous potassium dihydrogen phosphate a distilled ruwa, sa'an nan a gauraye a cikin distilled ruwa 100ml da 800mg EDTA disodium gishiri narkar da. Tsarma da ruwa mai tsafta zuwa 1L, zaɓin ƙara 20mg mercuric chloride ko digo 2 na toluene don hana ci gaban mold. Ƙara 20 MG na mercuric chloride zai iya kawar da tsangwama na adadin iodide wanda zai iya zama yayin auna chlorine kyauta. (Lura: Mercury chloride mai guba ne, rike tare da taka tsantsan kuma guje wa ciki)
N, N-diethyl-p-phenylenediamine (DPD) Nuni: Narke 1.5g DPD sulfate pentahydrate ko 1.1g anhydrous DPD sulfate a cikin chlorine-free distilled ruwa dauke da 8ml1 + 3 sulfuric acid da 200mg EDTA disodium gishiri, dilute zuwa 1 lita gishiri. a cikin kwalban gilashin ƙasa mai launin ruwan kasa, kuma adana a wuri mai duhu. Lokacin da alamar ta ɓace, yana buƙatar sake ginawa. A kai a kai duba ƙimar ɗaukar samfuran blank,
Idan ƙimar ɗaukar sarari a 515nm ta wuce 0.002/cm, ana buƙatar watsi da sake fasalin.
Potassium iodide (KI crystal)
Maganin Sodium arsenite: Narkar da 5.0g NaAsO2 a cikin ruwa mai narkewa kuma a tsoma shi zuwa lita 1. Lura: NaAsO2 yana da guba, kauce wa sha!
Maganin Thioacetamide: Narke 125 MG na thioacetamide a cikin 100 ml na ruwa mai narkewa.
Maganin Glycine: Narkar da 20g glycine a cikin ruwa maras chlorine kuma tsarma zuwa 100ml. Store daskararre. Ana buƙatar sake ginawa lokacin da turbidity ya faru.
Maganin sulfuric acid (kimanin 1mol/L): Narkar da 5.4ml maida hankali H2SO4 cikin 100ml distilled ruwa.
Maganin Sodium hydroxide (kimanin 2mol/L): Auna 8g NaOH kuma a narkar da shi a cikin ruwa mai tsabta 100ml.
Calibration (aiki) curve
Zuwa jerin bututu mai launi na 50, ƙara 0.0, 0.25, 0.50, 1.50, 2.50, 3.75, 5.00, 10.00ml na potassium iodate daidaitaccen bayani, bi da bi, ƙara game da 1g na potassium iodide da 0.5ml na sulfuric acid da sulfuric acid. tsaya na minti 2, sa'an nan kuma ƙara 0.5ml sodium hydroxide bayani da kuma tsarma zuwa alamar. Abubuwan da ke cikin kowace kwalban sun yi daidai da 0.00, 0.05, 0.10, 0.30, 0.50, 0.75, 1.00, da 2.00 mg/L na chlorine da ake samu. Ƙara 2.5ml na phosphate buffer da 2.5ml na DPD bayani mai nuna alama, gauraya da kyau, kuma nan da nan (a cikin minti 2) auna abin sha a 515nm ta amfani da 1-inch cuvette. Zana daidaitaccen lanƙwasa kuma nemo ma'auni na koma baya.
Matakan ƙaddara
Chlorine dioxide: Add 1ml na glycine bayani zuwa 50ml na ruwa samfurin da Mix, sa'an nan ƙara 2.5ml na phosphate buffer da 2.5ml na DPD bayani bayani, Mix da kyau, da kuma auna absorbance nan da nan (a cikin 2 minutes) (karanta ne G).
Chlorine dioxide da chlorine samuwa kyauta: Ɗauki wani samfurin ruwa na 50ml, ƙara 2.5ml phosphate buffer da 2.5ml DPD bayani bayani, Mix da kyau, da kuma auna abin sha nan da nan (a cikin minti 2) (karanta shine A).
7.3 Chlorine dioxide, chlorine samuwa kyauta da chlorine da aka haɗa: Ɗauki wani samfurin ruwa 50ml, ƙara game da 1g na potassium iodide, ƙara 2.5ml na phosphate buffer da 2.5ml na DPD bayani bayani, Mix da kyau, da kuma auna absorbance nan da nan (a ciki). Minti 2) (Karanta C ne).
Jimlar chlorine da aka samo ciki har da chlorine dioxide kyauta, chlorite, chlorine kyauta kyauta da kuma hadaddiyar chlorine: Bayan samun karatun C, ƙara 0.5ml sulfuric acid bayani a cikin samfurin ruwa a cikin kwalban launi iri ɗaya, kuma gauraya Bayan tsayawa har tsawon mintuna 2, ƙara. 0.5 ml sodium hydroxide bayani, Mix da kuma auna absorbance nan da nan (karanta ne D).
ClO2=1.9G (lasafta shi azaman ClO2)
Chlorine kyauta = AG
Haɗuwa akwai chlorine = CA
Jimlar chlorine=D
Chlorite=D-(C+4G)
Illar Manganese: Mafi mahimmancin abu mai shiga tsakani da ake fuskanta a cikin ruwan sha shine manganese oxide. Bayan ƙara phosphate buffer (4.3), ƙara 0.5 ~ 1.0ml sodium arsenite bayani (4.6), sa'an nan kuma ƙara alamar DPD don auna abin sha. Cire wannan karatun daga karatun A don kawar da shi
Cire tsangwama daga manganese oxide.
Tasirin zafin jiki: Daga cikin duk hanyoyin bincike na yanzu waɗanda zasu iya bambanta ClO2, chlorine kyauta da chlorine hade, gami da amperometric titration, ci gaba da hanyar iodometric, da dai sauransu, zafin jiki zai shafi daidaiton bambanci. Lokacin da zafin jiki ya fi girma, haɗin chlorine (chloramine) za a sa ya shiga cikin dauki a gaba, yana haifar da sakamako mafi girma na ClO2, musamman chlorine kyauta. Hanyar farko ta sarrafawa ita ce sarrafa zafin jiki. A kusa da 20 ° C, za ku iya ƙara DPD zuwa samfurin ruwa kuma ku gauraya shi, sa'an nan kuma ƙara 0.5ml thioacetamide bayani (4.7) don dakatar da haɗin chlorine (chloramine) daga DPD. Martani.
Tasirin lokacin launi: A gefe ɗaya, launin ja da ClO2 da DPD suka samar ba shi da kwanciyar hankali. Da duhun launi, da sauri ya ɓace. A daya hannun, kamar yadda phosphate buffer bayani da DPD nuna alama suna gauraye a kan lokaci, su da kansu ma za su shude. Yana samar da launin ja na karya, kuma kwarewa ta nuna cewa wannan rashin zaman lafiyar launi na lokaci shine babban dalilin rage yawan daidaiton bayanai. Don haka, hanzarta kowane matakin aiki yayin da ake sarrafa daidaitattun lokacin da aka yi amfani da shi a kowane mataki yana da mahimmanci don haɓaka daidaito. Dangane da gwaninta: haɓakar launi a ƙasa da 0.5 MG / L na iya zama tsayayye na kusan mintuna 10 zuwa 20, haɓakar launi a cikin taro na kusan 2.0 mg / L na iya zama barga kawai na kusan mintuna 3 zuwa 5, kuma Ci gaban launi a maida hankali sama da 5.0 mg/L zai kasance barga don ƙasa da minti 1.
TheSaukewa: LH-P3CLOA halin yanzu Lianhua tana samarwa ne mai ɗaukar hotoragowar chlorine mitawanda ya dace da hanyar DPD photometric.
Mai nazari ya riga ya saita tsayin daka da lankwasa. Kuna buƙatar ƙara reagents kawai da yin launi don samun saurin samun sakamakon ragowar chlorine, jimlar chlorine da chlorine dioxide cikin ruwa cikin sauri. Hakanan yana tallafawa samar da wutar lantarki da wutar lantarki na cikin gida, yana sauƙaƙa amfani da shi ko a waje ko a cikin dakin gwaje-gwaje.
Lokacin aikawa: Mayu-24-2024
